GSM6618574: Female, Lung, HDM, 30319-060; Mus musculus; miRNA-Seq - SRA

SRX17820015: GSM6618574: Female, Lung, HDM, 30319-060; Mus musculus; miRNA-Seq
1 ILLUMINA (NextSeq 550) run: 12.8M spots, 969.6M bases, 349Mb downloads

External Id: GSM6618574_r1

Submitted by: University of Nevada, Reno

Study: Gene network analysis for identification of microRNA biomarkers for asthma [Lung_miRNASeq]

PRJNA887770 • SRP401335 • All experiments • All runs

show Abstracthide Abstract

To analyze lung miRNA signatures using a house dust mite model (HDM) of asthma. microRNA sequencing (miRNA-seq) from BALB/c mice (male and female, ages 6-8 weeks old) lung tissue was performed. Overall design: Lung miRNA profile from HDM-exposed mice (n=16) was compared to the profile of Saline-treated (controls, n=14) mice.

Sample: Female, Lung, HDM, 30319-060

SAMN31184956 • SRS15343981 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6618574

Instrument: NextSeq 550

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: RNA was extracted with the RNeasy mini kit (Qiagen), quantified using a Nanodrop, and the RNA integrity was assessed using the Agilent TapeStation. miRNA libraries were prepared using Qiagen's miRNA Library Kit following the manufacturer's protocol.

Runs: 1 run, 12.8M spots, 969.6M bases, 349Mb

Run	# of Spots	# of Bases	Size	Published
SRR21830817	12,758,279	969.6M	349Mb	2022-12-26

ID:: 24746174

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